|本期目录/Table of Contents|

[1]谭乔燕 王权 旷梁 谢杨丽 李灿 罗凤涛 杜晓兰 陈林.小鼠关节软骨表层细胞分离培养及鉴定[J].国际骨科学杂志,2018,05:326.
　TAN Qiaoyan,WANG Quan,KUANG Liang,et al.Isolation, culture and identification of cells in the superficial zone of mouse articular cartilage[J].International Journal of Orthopaedics,2018,05:326.
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小鼠关节软骨表层细胞分离培养及鉴定(PDF)

《国际骨科学杂志》[ISSN:1673-7083/CN:31-1952/R]

期数:
2018年05期
页码:
326
栏目:
实验研究
出版日期:
2018-09-25

文章信息/Info

Title:
Isolation, culture and identification of cells in the superficial zone of mouse articular cartilage
作者:
谭乔燕 王权 旷梁 谢杨丽 李灿 罗凤涛 杜晓兰 陈林
400042 重庆, 陆军军医大学大坪医院全军战创伤中心创伤实验室(谭乔燕、王权、旷梁、谢杨丽、李灿、罗凤涛、杜晓兰、陈林)、骨代谢与修复中心(陈林)、创伤、烧伤、复合伤国家重点实验室(谢杨丽、杜晓兰、陈林)
Author(s):
TAN Qiaoyan1;  WANG Quan1;  KUANG Liang1;  XIE Yangli1; 3;  LI Can1;  LUO Fengtao1;  DU Xiaolan1; 3;  CHEN Lin1; 2; 3
Trauma Center, Research Institute of Surgery, Daping Hospital, Army Medical University1, Chongqing 400042, China; Center of Bone Metabolism and Repair2, Chongqing 400042, China; State Key Laboratory of Trauma, Burns and Combined Inju
关键词:
纤连蛋白类;  关节软骨;  干细胞;  细胞分化;  再生
Keywords:
Fibronectins;  Cartilage;  articular;  Stem cells;  Cell differentiation;  Regeneration
分类号:
-
DOI:
10.3969/j.issn.1673-7083.2018.05.014
文献标识码:
-
摘要:
目的 分离培养小鼠关节软骨表层细胞(ACSC)并鉴定其干细胞特性。方法 运用纤连蛋白黏附法从3～5 d新生小鼠膝关节软骨表层分离细胞及培养,对分离细胞以流式细胞仪检测干细胞表面阳性标志物(CD44与CD90)和阴性标志物(CD45、CD31与CD34)的表达; 实时荧光定量逆转录-聚合酶链反应(qRT-PCR)检测相关基因表达; 单克隆形成实验检测克隆形成能力。三系诱导分化(成软骨、成骨与成脂分化)检测分离培养细胞的多向分化潜能。取第6代分离培养细胞做裸鼠皮下移植实验,检测其体内成软骨能力。结果 纤连蛋白
Abstract:
Objective To isolate and culture mouse articular cartilage superficial zone cell(ACSC), and identify their characteristics.Methods Cells were isolated from mouse articular cartilage superficial zone by fibronectin-conglutination assay. The expression of p

参考文献/References


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备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展计划(2014CB942904)、国家自然科学基金(81530071)、国家自然科学基金(81472074)
作者单位: 400042 重庆, 陆军军医大学大坪医院全军战创伤中心创伤实验室(谭乔燕、王权、旷梁、谢杨丽、李灿、罗凤涛、杜晓兰、陈林)、骨代谢与修复中心(陈林)、创伤、烧伤、复合伤国家重点实验室(谢杨丽、杜晓兰、陈林)
通信作者: 陈林 E-mail: linchen70@163.com
更新日期/Last Update: 2018-09-25