|本期目录/Table of Contents|

[1]邵翔,陈后煌,陈达,等.透骨消痛胶囊抑制脂多糖诱导软骨细胞炎症反应的机制研究[J].国际骨科学杂志,2016,06:406-410.
　SHAO Xiang,CHEN Hou-huang,CHEN Da,et al.Mechanism of Tougu Xiaotong capsule inhibits lipopolysaccharide-induced inflammation in chondrocytes[J].International Journal of Orthopaedics,2016,06:406-410.
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透骨消痛胶囊抑制脂多糖诱导软骨细胞炎症反应的机制研究(PDF)

《国际骨科学杂志》[ISSN:1673-7083/CN:31-1952/R]

期数:
2016年06期
页码:
406-410
栏目:
实验研究
出版日期:
2016-11-25

文章信息/Info

Title:
Mechanism of Tougu Xiaotong capsule inhibits lipopolysaccharide-induced inflammation in chondrocytes
作者:
邵翔; 陈后煌; 陈达; 马玉环; 郑文伟; 叶蕻芝; 李西海
350122, 福建中医药大学中西医结合研究院(邵翔、陈后煌、陈达、李西海); 350122, 福建中医药大学药学院(马玉环、郑文伟); 350122, 福建省中西医结合老年性疾病重点实验室(叶蕻芝)
Author(s):
SHAO Xiang1;  CHEN Hou-huang1;  CHEN Da1;  MA Yu-huan2;  ZHENG Wen-wei2;  YE Hong-zhi3;  LI Xi-hai1.
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine1, Fuzhou 350122, China; College of Pharmacy, Fujian University of Traditional Chinese Medicine2, Fuzhou 350122, China; Fujian Key Laboratory of Integr
关键词:
软骨细胞;  微小RNA-140;  骨关节炎;  基质金属蛋白酶-3;  炎症反应
Keywords:
Chondrocyte;  microRNA-140;  Osteoarthritis;  Matrix metalloproteinase-3;  Inflammation
分类号:
-
DOI:
10.3969/j.issn.1673-7083.2016.06.014
文献标识码:
-
摘要:
目的 探讨透骨消痛胶囊抑制脂多糖(LPS)诱导的软骨细胞炎症反应作用机制。方法 对4周龄雄性SD大鼠采用机械-Ⅱ型胶原酶消化法获取膝关节软骨细胞并进行体外培养,将软骨细胞分为空白组、模型组以及透骨消痛胶囊组,空白组加入含10%胎牛血清(FBS)的低糖型Dulbecco改良Eagle培养基(DMEM/LOW)2 mL,模型组加入含10 ng/mL LPS、10%FBS的DMEM/LOW培养基 2 mL,透骨消痛胶囊组加入含10 ng/mL LPS、透骨消痛胶囊(300 μg/mL)、10%FBS的DMEM/LOW培养基 2 mL。干预8 h后,实时荧光定量聚合酶链式反应(qPCR)检测各组微小RNA(miRNA)-140表达变化,免疫荧光观察各组带有血小板凝血酶敏感蛋白样模体的解整链蛋白金属蛋白酶(ADAMTS)4、基质金属蛋白酶(MMP)-3表达变化,Image J分析各组平均光密度。结果 与空白组相比,模型组MMP-3表达升高(P<0.05)、ADAMTS4表达明显升高(P<0.01); 与模型组相比,透骨消痛胶囊组MMP-3表达下降(P<0.05)、ADAMTS4表达明显下降(P<0.01)。与空白组相比,模型组软骨细胞miRNA-140表达明显降低(P<0.05); 与模型组相比,透骨消痛胶囊组软骨细胞miRNA表达明显升高(P<0.05)。结论 透骨消痛胶囊能促进miRNA-140表达,降低ADAMTS4、MMP-3分泌,抑制炎症反应介导的软骨基质降解,从而延缓骨关节炎软骨退变。
Abstract:
Objective To explore the mechanism of Tougu Xiaotong capsule(TXC)inhibiting lipopolysaccharide(LPS)-stimulated inflammation in chondrocytes.Methods The chondrocytes harvested from the knee joints of 4-week-old SD rats were cultured in vitro and divided in

参考文献/References

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金面上项目(81573998)、福建省自然科学基金(2016J01395)、福建省中青年教师教育科研项目(科技A类)优秀人才(JA14150)作者单位: 350122, 福建中医药大学中西医结合研究院(邵翔、陈后煌、陈达、李西海); 350122, 福建中医药大学药学院(马玉环、郑文伟); 350122, 福建省中西医结合老年性疾病重点实验室(叶蕻芝)通信作者: 李西海 E-mail: lixihai79dahai@163.com
更新日期/Last Update: 2016-11-20